Chapter 22 Precision Cutting

After the preparations were completed, Cheng Xiang concentrated on summoning the system. After preliminary research, this task must be completed quickly. The experience value invested in the knowledge ball will be more than 12,300 points, and the fragmented knowledge involved Cheng Xiang carefully selected the number and determined two hundred and forty-eight.

There are two hundred and forty-eight knowledge points, about a quarter of which are distributed in basic biological theory, and the rest are all knowledge fragments under the basic gene editing knowledge node.

"Two hundred and forty-eight. This time, we must insist on at least forty, and try to hold on to fifty. If possible, we must give sixty a try." Cheng Xiang clenched his fist tightly.

Just do it. Some things, if you hesitate for a long time, you will easily lose courage.

Grabbing a piece of cooked beef in one hand, Cheng Xiang began the difficult journey of lighting up. This time's knowledge enlightenment mainly focuses on the deep embedding technology of genes.

The difference between the deep gene embedding technology and the current transgenic technology in genetic engineering is not only reflected in flexibility and success rate. The bigger difference is that the current transgenic technology is full of uncertainties and is a very crude and barbaric method. The deep gene embedding technology has opened a new door in the field of genetic modification. Not only can the manipulation of genes be made more precise and flexible, but the traits expressed by genes can also be combined more freely.

"Lighting up the DNA cutting enzyme code..."

"Lighting up the precise positioning of genes..."

"Light up..."

…

Time has never been so stagnant. More than four hours of torture and tearing pain are enough to make people collapse every minute. However, Cheng Xiang managed to survive. There was a large pool of water stains on the ground, all made up of sweat from the body.

At this time, Cheng Xiang was lying in the middle of a pile of food waste as if he had fallen apart, with a silly smile on his face.

"Haha, six...ten...four, I...win...again."

Repeatedly, five days passed in the blink of an eye.

In five days and three times, Cheng Xiang lit up a total of 248 pieces of knowledge.

After a full day's rest, Cheng Xiang reappeared in the research institute building, full of energy.

"Cheng Xiang, you are here and I am about to report to you." Chen Wenli said.

"Tell me about it." Cheng Xiang went straight to the point.

"The entire sample processing has been completed and the culture medium has been produced."

Cheng Xiang smiled when he heard this, "The speed is pretty good, let's go take a look."

"Okay." Chen Wenli walked side by side and said as she walked, "In the past two days, we have initially screened out samples that are sensitive to formaldehyde, TVOC and benzene compounds."

"Well, what then?"

"Next we tried to extract the target gene, but it was very unsuccessful. Except for a small number of reference samples that were successful, in the remaining samples, some of the gene fragments we needed could not locate the target gene, and some We can locate it, but we still can't find a suitable cutting enzyme. We are still trying." Chen Wenli frowned in distress, some things are easier said than done.

The book says that you can extract gene fragments and cut them with restriction enzymes, but how to find the target gene and what kind of enzyme can successfully cut it is another matter.

This process usually relies on experience or literature. For those uncommon gene fragments, if you are lucky, you may find them right away. If you are not lucky, it depends on your reputation. If you have a bad face, I'm afraid it will be difficult.

"Don't be discouraged. This is normal. It is easy to encounter this kind of thing during gene cutting."

They walked to the laboratory and entered the changing room. After changing clothes, cleaning and disinfecting them, the two walked into the laboratory.

Some members in the laboratory were already busy. Seeing Cheng Xiang coming, they all nodded in greeting.

Cheng Xiang smiled and nodded in response one by one, and then immediately began to intervene in the questions raised by Chen Wenli. After looking at the samples where Chen Wenli failed to extract genes one by one, Cheng Xiang already knew very well what was needed in the current experimental stage.

"Let me see the genetic sample you successfully extracted." Cheng Xiang wanted to confirm.

"Okay." Chen Wenli worked very efficiently.

Ten minutes later, Cheng Xiang looked up from the electron microscope. The successfully extracted genes were also briefly reviewed.

"How is it?" Although many samples were not successfully extracted, Chen Wenli felt that it was pretty good to be able to do so much in such a short time.

Cheng Xiang glanced at Chen Wenli and said with a smile, "Do you want to hear the truth or a lie?"

"Why are you like this?" Chen Wenli pouted, "Do we who do scientific research need someone who listens well?"

"That's good, then I'll say it straight." Cheng Xiang responded.

"Yeah." When Chen Wenli heard Cheng Xiang say this, she suddenly felt a little unconfident.

"Generally speaking, it's pretty good to be able to do this, though."

"But what?" Chen Wenli asked.

"However, in our project, the extraction of target genes does not meet the requirements, or in other words, it only reaches a preliminary screening state."

"Preliminary screening?" Chen Wenli was a little confused.

"That's right, it's the preliminary screening. We'll go to the office and talk about it."

"good."

Cheng Xiang understands Chen Wenli's doubts very well, because the requirement to achieve precise gene cutting is, after all, out of line. In other words, gene extraction in contemporary genetic engineering is what Chen Wenli is currently doing. Find the gene segment that needs to be extracted, and then choose the appropriate one. Enzyme cuts it off.

As long as the cut is not too small, it is fine. As for whether the cut is too much, there are no such strict requirements. Because there is no corresponding technical means to achieve it.

“The gene fragments we need to extract for our project need to be precise,” Cheng Xiang reminded.

"Accurate?" Chen Wenli realized something, her eyebrows narrowed, and then she asked, "How accurate should it be?"

"To what extent?" Cheng Xiang smiled slightly, "Of course, it is as accurate as possible. One more deoxyribonucleotide is not enough, and one less deoxynucleoside is not enough."

"Ah!" Chen Wenli was very surprised. She had thought of many answers, but she never expected that Cheng Xiang would give such an answer, "Cheng Xiang, are you kidding me?"

"Is this possible?" Chen Wenli asked.

"Okay." Cheng Xiang replied affirmatively.

"Really?" Chen Wenli still didn't believe it.

"Of course it's true." Cheng Xiang nodded seriously.

"But how is this possible? You must be joking, right." Chen Wenli couldn't accept it and stopped in her tracks. It has been almost eight years since she studied biology and became a Ph.D. What is the state of contemporary gene extraction? Where is she? Can't be clear. The more clear it is, the more difficult it is to accurately cut genes. The more we understand the difficulty, the more unbelievable it becomes.

In the field of contemporary genetic engineering, not everyone has not thought about cutting more accurately, but it is really too difficult. It’s hard to believe that despite decades of promotion by the biological science community, the threshold for precise cutting has not yet been reached.

"Cleaving deoxyribonucleic acid as accurately as possible, if Cheng Xiang can really do it." Chen Wenli didn't dare to think about it anymore. Seeing Cheng Xiang walking away, Chen Wenli hurriedly chased after him.

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