Vol 2 Chapter 1910: cloned cells

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"In addition, these special cell preservation solutions can keep the cells to be printed at a low temperature all the time, so that the whole cells are in a dormant and extremely low activity state. This is conducive to prolonging the preservation period of these cells, for the future. 3D printing buys time.

Of course, using liquid to store and transport cells has advantages and disadvantages. The biggest disadvantage is that these liquids are attached to these tiny cells. How to remove excess water from these cells before printing without damaging the cells? This is a very important problem. technical problems to be solved.

Furthermore, these cells to be printed are in the millions and millions. In such a large base, there will inevitably be some anomalies, such as necrotic cells, mutated cells, and cells of the opposite sex. How to remove these cells and prevent these bad cells from being printed into organs and tissues is also an urgent technical problem that needs to be solved.

In the past, trying to solve this problem was basically impossible. And now with the help of artificial intelligence systems, we can monitor the status of these cells at all times as they are being transported. And through the tiny probes in time, the bad cells mixed in these huge cell groups can be accurately sucked out to ensure that the cells used for printing are all healthy cells. "

Speaking of this, Wu Hao breathed a sigh of relief, and then said with a smile: "After solving such a series of problems, the next step is the printing process. How to glue these different cell combinations together is also a problem that we need to solve.

Use hot melt stacking, or light curing? "

Wu Hao smiled and shook his head: "Cells are alive, how to stack these cells in an orderly combination, whether it is hot-melt stacking or light curing is obviously not suitable, which requires a new printing technology.

As we all know, we humans generally need several basic processes for wound healing. The first is the acute inflammatory stage. In the early changes of the wound, there are different degrees of tissue necrosis and blood vessel rupture and bleeding in the local wound, and an inflammatory reaction occurs within a few hours. As a result, congestion, serous exudation and leukocyte exudation occur, resulting in local redness and swelling. Subsequently, the fibrinogen in the blood and fluid exuding from the wound quickly coagulates to form a clot, forming a crust on the surface, which can stop bleeding, isolate and protect the wound, and prevent infection.

Next is the cell growth period. After the wound shrinks for two or three days, the entire layer of skin and subcutaneous tissue at the edge of the wound moves to the center, so the wound shrinks rapidly until it stops in about two weeks.

The proliferation and scarring of granulation tissue begins approximately on day 3 after injury, with granulation tissue growing from the bottom and edges of the wound to fill the wound. Fibroblasts produce collagen fibers from five or six days, and the formation of collagen fibers is very active in the following week, and then gradually slows down. As more and more collagen fibers become available, a scarring process occurs, and the scarring is fully formed about a month after the injury. Possibly due to the effect of local tension, the collagen fibers in the scar end up parallel to the skin surface.

Therefore, in the process of organ tissue printing, we also need to simulate the process of wound healing and apply a biogel based on collagen research, which can evenly adhere these cells together, and then be absorbed by the cells evenly. , there will be no residue.

In this way, after continuous printing, we obtained a desired complete 3D bioprinted organ tissue. "

"Simple, it seems to be very simple, but in fact there are still many problems to be solved. For example, we seem to have overlooked a problem, that is, how do the cells used for organ tissue printing come from?"

Hearing this question, all the people watching the live broadcast began to talk about it curiously, indeed. How do these somatic cells for printing come from? There are many guesses, some say that it was directly excavated and dissolved from the patient, some said that it was obtained from a donor, and some said that it was made by oneself.

Wu Hao smiled and then replied: "In terms of the acquisition of cell sources, there have always been differences within our scientific research team. Some people believe that we should screen out a pluripotent cell population to undergo continuous cloning, cultivation and division. Finally, the printing-specific biological cells we need are formed.

However, there will be a problem in this way, that is, the printed organ tissue is not the patient's own, and implantation in the patient will produce a rejection reaction. This requires the patient to take medicine continuously, and the lifespan of such organs is generally not long, and the recovery effect is average, which cannot allow the patient to truly recover.

So other people think, why don't we just take the tissue from the patient and then clone it. Organ tissue printed with these cells will be implanted in patients without rejection, and the recovery will be good, allowing patients to basically return to normal life.

Finally, after constant discussion and research, we chose the second method. Although it is more difficult and technically demanding, it can better cure patients and bring health to patients.

In this way, we have to solve the cloning technology of this somatic cell. Again, the technology seems simple enough. After all, in everyone's cognition, cloning technology seems to have existed decades ago, so there should be no problem.

But the cloning technology we all know is very different from the cloning technology we need. We cannot cultivate with the help of the mother, which will bring a series of social moral, legal and legal problems. So we can only carry out in vitro cloning cultivation, which requires us to develop an 'artificial Z palace' or 'artificial placenta'.

And this device must have sufficient performance, UU reading www. uukanshu.com can clone and cultivate enough cells in the shortest time.

As we all know, patients undergoing organ transplantation are generally in critical condition, so there is not much time to wait. This requires us, whether it is cell clone cultivation or bio-3D printing, to be controlled within a very short period of time.

However, the division and reproduction of cells, including clone cultivation, take time, and the division and growth time of this cell can be accelerated by special hormone drugs. But doing so can also be harmful, and the printed organs and tissues can also have problems, which can be dangerous to patients when implanted.

Therefore, a new cell clone cultivation technology is needed, which can allow cells to divide and grow rapidly and healthily, so as to obtain enough cloned cells in the shortest time.

In order to develop this technology, we have invested a lot of financial resources and human and material resources, and started a long road of scientific research and exploration in cooperation with domestic and international famous scientific research institutions and laboratories.

And invited relevant scientific research experts, as well as relevant experts from the hospital to conduct research, and finally obtained a set of feasible research plans. "